Systemtheorie - Methoden und Anwendungen für ein- und mehrdimensionale Systeme (German Edition)

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Ambos formatos presentan distintas ventajas. Ventajas para los ciudadanos: Ventajas para los gobiernos: Se establece un mayor nivel de confianza entre las entidades del gobierno y el titular de la tarjeta. Debe ser un proceso voluntario: El director general de SoftGuard, Daniel Banda, dijo al respecto: Seguridad personal, la prioridad dentro de cualquier proceso productivo Las lesiones en el lugar de trabajo causan tiempo de inactividad y disminuyen la productividad.

Cuando los paquetes caen de una cinta transportadora o. NO son una amenaza, sino todo lo contrario, una gran. Pero no fue hasta , en Filadelfia, cuando Frederick Graft Sr. Se usa el que sea mayor de los dos. Moncada es jam ifsc. Se debe proteger el cuarto de control por piso y techo, con igual nivel de resistencia. Las puertas cortafuego de un nivel de seguridad importante y salidas de emergencia siguiendo las normas NFPA. Se deben colocar varias tomas en los sitios para posibles bancos de prueba para mantenimiento. En el resto de espacios complementarios se requieren al menos 2 tomas tradicionales.

Piso falso, Techo falso, Apantallamiento Solo si el cuarto de control es grande, o se espera gran densidad de equipos, se recomienda un piso falso a 30 cmts de altura, especialmente en las zonas de equipos y parte de la zona de operadores. Cuarto de control alterno. Un segundo operador de tele comunicaciones casi siempre es utilizado, minimizando las posibilidades de fallo.

La idea de base era reemplazar los grandes. Este modelo cambia completamente el funcionamiento de los bancos tal como se conocen en la actualidad. Una moneda digital o criptomoneda independiente de gobiernos, estados y bancos. En todo el mundo las monedas dependen de un banco central o un estado controlador de sus operaciones. Se registra el dominio Bitcoin. Proliferan las plataformas en las que se puede comprar y vender divisa virtual.

Se acepta el primer pago comercial de Bitcoin. Ese mismo mes, un usuario paga Se celebra una conferencia en Londres en torno a la moneda virtual. Wikileaks comienza a aceptar pagos en bitcoins. Se celebra en Praga la primera gran conferencia internacional en torno a Bitcoin. Cierra Tradehill, el primer gran mercado de transacciones de Bitcoin por problemas regulatorios y por una disputa entre sus usuarios. En la recta final de Las agencias especializadas en blanqueo de capitales ponen el foco en el sistema bitcoin.

El fundador de Bitinstant y presidente. Gox, el mayor mercado de bitcoins. El ecosistema empieza a profesionalizarse. Viendo el historial de sucesos, al formalizarse de a poco Bitcoin surgen nuevas monedas competidoras de distintas empresas. Su nombre hace referencia a PPCoin o Peerwww. Since the ribosome is an essential organelle that is present in large numbers in all living cells, this ability of the ribosome provides an energetically inexpensive way to suppress cellular aggregation.

Further, this ability of the ribosome might also be crucial in the context that the ribosome is one of the first chaperones to be encountered by a large nascent polypeptide chains that have a tendency to form partially folded intermediates immediately following their synthesis. Se identificaron dos clones recombinantes cof-ubi1 y cof-ubi2 que hibridizaron con la sonda tipo ubiquitina.

The ribosome is the quintessential antibacterial drug target, with many structurally and mechanistically distinct classes of antibacterial agents acting by inhibiting ribosome function. Detecting and quantifying ribosome inhibition by small molecules and investigating their binding modes and mechanisms of action are critical to antibacterial drug discovery and development efforts.

To develop a ribosome inhibition assay that is operationally simple, yet provides direct information on the drug target and the mechanism of action, we have developed engineered E. As a proof of concept, we demonstrate that these strains, when coexpressing homogeneous populations of aminoglycoside resistant ribosomes , act as sensitive and quantitative detectors of ribosome inhibition by a set of 12 structurally diverse aminoglycoside antibiotics. We suggest that this strategy can be extended to quantifying ribosome inhibition by other drug classes. Effect of primary and secondary radicals on chain breaks in ribosomal RNA in E.

It has been shown previously that, in dilute aerated solutions, ribosomes are inactivated by OH radicals and by secondary radicals produced from added alcohols Singh and Vadasz a. In de-aerated solutions, both radicalH and e - sub aq also inactivate ribosomes Singh and Vadasz b. The results of these studies and other on different systems Adams et al. The purpose of this study was to determine which of the primary and secondary radicals cause chain breaks in ribosomal RNA rRNA within the ribosomes.

GTPases and the origin of the ribosome. Full Text Available Abstract Background This paper is an attempt to trace the evolution of the ribosome through the evolution of the universal P-loop GTPases that are involved with the ribosome in translation and with the attachment of the ribosome to the membrane. Results 1 The Elongation Factors of the Archaea based on structural considerations of the domains have the following evolutionary path: The evolution of the aeIF5b was a later event; 2 the Elongation Factors of the Bacteria based on the topological considerations of the GTPase domain have a similar evolutionary path: Conclusion The evolution of translational GTPases of both the Archaea and Bacteria point to the evolution of the ribosome.

Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site. Translation initiation can occur by multiple pathways. To delineate these pathways by single-molecule methods, fluorescently labeled ribosomal subunits are required. The resulting ribosomal subunits could be specifically labeled in living cells and in vitro. We furthermore demonstrated that after binding, the 40S: Addition of translation extracts suppresses these fluctuations, funneling the complex into a single conformation on the 80S assembly pathway.

These findings show that 40S: HCV IRES complex formation is accompanied by dynamic conformational rearrangements that may be modulated by initiation factors. Produced water from offshore oil platforms is a major source of oil and related chemicals into the sea. The large volume and high salinity of produced water could pose severe environmental impacts upon inadequate disposal. This study is based on direct field sampling of effluents released into the ocean in the years and at the Sonda de Campeche located in the southern part of the Gulf of Mexico.

Metals and hydrocarbons were characterized in water, sediments, and fish tissues at the discharge site and compared with those obtained at two reference sites. Chemicals that exceeded risk-based concentrations in the discharge included the metals As, Pb, Cd, and Cr, and a variety of compounds polycyclic aromatic hydrocarbon PAHs , including naphthalene, fluorenes, and low molecular weight PAHs. The values of low to high molecular weight polycyclic aromatic hydrocarbon PAHs , and carbon preference index indicate that hydrocarbons in sediments of the discharge zone are originated from the produced water and combustion sources.

Results suggest that, from to , discharges of produced water have had a non-negligible impact on ecosystems at a regional level, so the possibility of subtle, cumulative effects from operational discharges should not be ignored. Ribosomal history reveals origins of modern protein synthesis. Full Text Available The origin and evolution of the ribosome is central to our understanding of the cellular world.

Most hypotheses posit that the ribosome originated in the peptidyl transferase center of the large ribosomal subunit. However, these proposals do not link protein synthesis to RNA recognition and do not use a phylogenetic comparative framework to study ribosomal evolution. Here we infer evolution of the structural components of the ribosome. Phylogenetic methods widely used in morphometrics are applied directly to RNA structures of thousands of molecules and to a census of protein structures in hundreds of genomes.

We find that components of the small subunit involved in ribosomal processivity evolved earlier than the catalytic peptidyl transferase center responsible for protein synthesis. Remarkably, subunit RNA and proteins coevolved, starting with interactions between the oldest proteins S12 and S17 and the oldest substructure the ribosomal ratchet in the small subunit and ending with the rise of a modern multi-subunit ribosome.

Ancestral ribonucleoprotein components show similarities to in vitro evolved RNA replicase ribozymes and protein structures in extant replication machinery. Our study therefore provides important clues about the chicken-or-egg dilemma associated with the central dogma of molecular biology by showing that ribosomal history is driven by the gradual structural accretion of protein and RNA structures. Most importantly, results suggest that functionally important and conserved regions of the ribosome were recruited and could be relics of an ancient ribonucleoprotein world.

Phosphorylation of acidic ribosomal proteins from rabbit reticulocytes by a ribosome -associated casein kinase. Two acidic proteins from S ribosomes were isolated and purified to homogeneity. The proteins became phosphorylated in situ, too.

Sodium dodecyl sulfate polyacryl The ribosomal protein Rpl22 controls ribosome composition by directly repressing expression of its own paralog, Rpl22l1. Full Text Available Most yeast ribosomal protein genes are duplicated and their characterization has led to hypotheses regarding the existence of specialized ribosomes with different subunit composition or specifically-tailored functions.

In yeast, ribosomal protein genes are generally duplicated and evidence has emerged that paralogs might have specific roles. Unlike yeast, most mammalian ribosomal proteins are thought to be encoded by a single gene copy, raising the possibility that heterogenous populations of ribosomes are unique to yeast. Consistent with the hypothesis that either ribosomal protein can support translation, knockdown of Rpl22l1 impairs growth of cells lacking Rpl Mechanistically, Rpl22 regulates Rpl22l1 directly by binding to an internal hairpin structure and repressing its expression.

We propose that ribosome specificity may exist in mammals, providing evidence that one ribosomal protein can influence composition of the ribosome by regulating its own paralog. Architecture of the large subunit of the mammalian mitochondrial ribosome. Mitochondrial ribosomes synthesize a number of highly hydrophobic proteins encoded on the genome of mitochondria, the organelles in eukaryotic cells that are responsible for energy conversion by oxidative phosphorylation. The ribosomes in mammalian mitochondria have undergone massive structural changes throughout their evolution, including ribosomal RNA shortening and acquisition of mitochondria-specific ribosomal proteins.

Here we present the three-dimensional structure of the 39S large subunit of the porcine mitochondrial ribosome determined by cryo-electron microscopy at 4. The structure, combined with data from chemical crosslinking and mass spectrometry experiments, reveals the unique features of the 39S subunit at near-atomic resolution and provides detailed insight into the architecture of the polypeptide exit site.

This region of the mitochondrial ribosome has been considerably remodelled compared to its bacterial counterpart, providing a specialized platform for the synthesis and membrane insertion of the highly hydrophobic protein components of the respiratory chain. Placeholder factors in ribosome biogenesis: Full Text Available The synthesis of cytoplasmic eukaryotic ribosomes is an extraordinarily energy-demanding cellular activity that occurs progressively from the nucleolus to the cytoplasm.

In the nucleolus, precursor rRNAs associate with a myriad of trans-acting factors and some ribosomal proteins to form pre- ribosomal particles. Their coordinate activity orchestrates in a spatiotemporal manner the modification and processing of precursor rRNAs, the rearrangement reactions required for the formation of productive RNA folding intermediates, the ordered assembly of the ribosomal proteins, and the export of pre- ribosomal particles to the cytoplasm; thus, providing speed, directionality and accuracy to the overall process of formation of translation-competent ribosomes.

Placeholder factors temporarily bind selected ribosomal sites until these have achieved a structural context that is appropriate for exchanging the placeholder with another site-specific binding factor. By this strategy, placeholders sterically prevent premature recruitment of subsequently binding factors, premature formation of structures, avoid possible folding traps, and act as molecular clocks that supervise the correct progression of pre- ribosomal particles into functional ribosomal subunits.

We summarize the current understanding of those factors that delay the assembly of distinct ribosomal proteins or subsequently bind key sites in pre- ribosomal particles. The architecture of mammalian ribosomal protein promoters. Full Text Available Abstract Background Mammalian ribosomes contain 79 different proteins encoded by widely scattered single copy genes. Coordinate expression of these genes at transcriptional and post-transcriptional levels is required to ensure a roughly equimolar accumulation of ribosomal proteins. To date, detailed studies of only a very few ribosomal protein rp promoters have been made.

To elucidate the general features of rp promoter architecture, I made a detailed sequence comparison of the promoter regions of the entire set of orthologous human and mouse rp genes. Results A striking evolutionarily conserved feature of most rp genes is the separation by an intron of the sequences involved in transcriptional and translational regulation from the sequences with protein encoding function. A remarkably high proportion of the promoters contain conserved binding sites for transcription factors that were previously implicated in rp gene expression, namely upstream GABP and Sp1 sites and downstream YY1 sites.

Conclusions This analysis has provided some valuable insights into the general architecture of mammalian rp promoters and has identified parameters that might coordinately regulate the transcriptional activity of certain subsets of rp genes. We estimated the influence of the temperature and concentration of molecules on the hopping probability used in the ASEP model. Our model can also treat environmental effects on the translation process that cannot be explained by such cellular automaton models.

As molecular phylogeny increasingly shapes our understanding of organismal relationships, no molecule has been applied to more questions than have ribosomal RNAs. We review this role of the rRNAs and some of the insights that have been gained from them. We also offer some of the practical considerations in extracting the phylogenetic information from the sequences. Finally, we stress the importance of comparing results from multiple molecules, both as a method for testing the overall reliability of the organismal phylogeny and as a method for more broadly exploring the history of the genome.

Eukaryotic ribosome display with in situ DNA recovery. Ribosome display is a cell-free display technology for in vitro selection and optimisation of proteins from large diversified libraries. It operates through the formation of stable protein- ribosome -mRNA PRM complexes and selection of ligand-binding proteins, followed by DNA recovery from the selected genetic information.

Both prokaryotic and eukaryotic ribosome display systems have been developed. In this chapter, we describe the eukaryotic rabbit reticulocyte method in which a distinct in situ single-primer RT-PCR procedure is used to recover DNA from the selected PRM complexes without the need for prior disruption of the ribosome. Ribosomes are the mediators of protein synthesis in the cell and therefore crucial to proper cell function. A large amount of resources go into maintaining this pool of ribosomes , so ribosome.


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Em janeiro de , foram coletadas amostras de solo com estrutura preservada a diferentes profundidades, nas linhas de plantio do cafeeiro e nas entrelinhas. A computational investigation on the connection between dynamics properties of ribosomal proteins and ribosome assembly.

DROGAS DIGITALES AUDIOS QUE TRASTORNAN

Full Text Available Assembly of the ribosome from its protein and RNA constituents has been studied extensively over the past 50 years, and experimental evidence suggests that prokaryotic ribosomal proteins undergo conformational changes during assembly. However, to date, no studies have attempted to elucidate these conformational changes.

The present work utilizes computational methods to analyze protein dynamics and to investigate the linkage between dynamics and binding of these proteins during the assembly of the ribosome. Ribosomal proteins are known to be positively charged and we find the percentage of positive residues in r-proteins to be about twice that of the average protein: Also, positive residues constitute a large proportion of RNA contacting residues: This affirms the known importance of charge-charge interactions in the assembly of the ribosome.

We studied the dynamics of three primary proteins from E. Molecular dynamics simulations show that solvent-exposed proteins S15 and S17 tend to adopt more stable solution conformations than an RNA-embedded protein S We also find protein residues that contact the 16S rRNA are generally more mobile in comparison with the other residues. This is because there is a larger proportion of contacting residues located in flexible loop regions. By the use of elastic network models, which are computationally more efficient, we show that this trend holds for most of the 30S r-proteins.

Avaliar o efeito de potenciais lubrificantes sobre a integridade da sonda de Foley. Os lubrificantes foram testados quanto a hidrossolubilidade. Vinte balonetes romperam-se, dos grupos vaselina e uma pomada. Nasal packing for epistaxis is usually carried out using lubricated gauze and a Foley catheter. Lubricants such as Vaseline or paraffin can dissolve the Foley balloon of latex; however, they are excipients of various ointments and creams. To evaluate the effect of potential lubricants on the Foley catheter. Balloons from eighty Foley catheters were distributed into groups of equal size and kept under traction in contact with one of eight different products: Defining the bacteroides ribosomal binding site.

The human gastrointestinal tract, in particular the colon, hosts a vast number of commensal microorganisms. Representatives of the genus Bacteroides are among the most abundant bacterial species in the human colon. Bacteroidetes diverged from the common line of eubacterial descent before other eubacterial groups.

As a result, they employ unique transcription initiation signals and, because of this uniqueness, they require specific genetic tools. Although some tools exist, they are not optimal for studying the roles and functions of these bacteria in the human gastrointestinal tract. Focusing on translation initiation signals in Bacteroides, we created a series of expression vectors allowing for different levels of protein expression in this genus, and we describe the use of pepI from Lactobacillus delbrueckii subsp.

Furthermore, we report the identification of the 3' end of the 16S rRNA of Bacteroides ovatus and analyze in detail its ribosomal binding site, thus defining a core region necessary for efficient translation, which we have incorporated into the design of our expression vectors. Based on the sequence logo information from the 5' untranslated region of other Bacteroidales ribosomal protein genes, we conclude that our findings are relevant to all members of this order. Mechanism of recycling of post-termination ribosomal complexes in RRF binding results in a remarkable conformational change.

Expression of protein-coding genes embedded in ribosomal DNA. Interestingly, rDNAs are frequently interrupted by parasitic elements, some of which carry protein genes. We describe in this paper, based on already published articles, a contribution to the theory postulating the existence of a proto- ribosome , which could have appeared early at the origin of life and we discuss the interest of this notion in an evolutionary perspective, taking into account the existence of possible RNA relics of this proto- ribosome. Full Text Available The ribosome carries out the synthesis of proteins in every living cell.

It consequently represents a frontline target in anti-microbial therapy. Tuberculosis ranks among the leading causes of death worldwide, due in large part to the combination of difficult-to-treat latency and antibiotic resistance. Here, we present the 3. The structure reveals two additional ribosomal proteins and localizes them to the vicinity of drug-target sites in both the catalytic center and the decoding site of the ribosome. Furthermore, we visualized actinobacterium-specific rRNA and protein expansions that extensively remodel the ribosomal surface with implications for polysome organization.

Our results provide a foundation for understanding the idiosyncrasies of mycobacterial translation and reveal atomic details of the structure that will facilitate the design of anti-tubercular therapeutics. Macrolide antibiotic interaction and resistance on the bacterial ribosome. Our understanding of the fine structure of many antibiotic target sites has reached a new level of enlightenment in the last couple of years due to the advent, by X-ray crystallography, of high-resolution structures of the bacterial ribosome.

Many classes of clinically useful antibiotics bind to the ribosome to inhibit bacterial protein synthesis. Macrolide, lincosamide and streptogramin B MLSB antibiotics form one of the largest groups, and bind to the same site on the 50S ribosomal subunit. Here, we review the molecular details of the ribosomal MLSB site to put into perspective the main points from a wealth of biochemical and genetic data that have been collected over several decades.

The information is now available to understand, at atomic resolution, how macrolide antibiotics interact with their ribosomal target, how the target is altered to confer resistance, and in which directions we need to look if we are to rationally design better drugs to overcome the extant resistance mechanisms. Cis-regulatory RNA elements that regulate specialized ribosome activity.

Recent evidence has shown that the ribosome itself can play a highly regulatory role in the specialized translation of specific subpools of mRNAs, in particular at the level of ribosomal proteins RP. However, the mechanism s by which this selection takes place has remained poorly understood. The unique combination of an inhibitor of general translation and an activator of specialized translation is key to ribosome -mediated control of gene expression.

Here we discuss how these RNA regulatory elements provide a new level of control to protein expression and their implications for gene expression, organismal development and evolution. Control of Ribosome Synthesis in Escherichia coli. The rate of ribosome synthesis and accumulation in Escherichia coli during the transition after an energy source shift-down was analyzed.

In steady states of growth with growth rates ranging from 0. The rate of synthesis of r RNA correlated during this transition — in contrast to the rate of accumulation M. Hierarchical recruitment of ribosomal proteins and assembly factors remodels nucleolar preS ribosomes. Ribosome biogenesis involves numerous preribosomal RNA pre-rRNA processing events to remove internal and external transcribed spacer sequences, ultimately yielding three mature rRNAs. C 2 cleavage requires the hierarchical recruitment of 11 ribosomal proteins and 14 ribosome assembly factors. However, the function of these proteins in C 2 cleavage remained unclear.

In this study, we have performed a detailed analysis of the effects of depleting proteins required for C 2 cleavage and interpreted these results using cryo-electron microscopy structures of assembling 60S subunits. This work revealed that these proteins are required for remodeling of several neighborhoods, including two major functional centers of the 60S subunit, suggesting that these remodeling events form a checkpoint leading to C 2 cleavage.

Interestingly, when C 2 cleavage is directly blocked by depleting or inactivating the C 2 endonuclease, assembly progresses through all other subsequent steps. The corn is a very important product, its production and comercialization is very intense, and besides it is too much nutritive. It is very important to know the thermophysical properties of the corn so that it can be stored and processed. In this work it was measured the thermal conductivity of the triturated corn and the relationship among the moisture content, the compactation level and the thermal conductivity was checked.

It was used the linear probe method, aplying a known heat quantity and measuring the time and the temperature variation in definited time interval. A comparative study of ribosomal proteins: Assembly of the ribosome from its protein and RNA constituents must occur quickly and efficiently in order to synthesize the proteins necessary for all cellular activity.

We utilize a comparative analysis to investigate the amino acid composition of ribosomal proteins r-proteins with respect to their role in the assembly process. We compared small subunit 30S r-protein sequences to those of other housekeeping proteins from bacterial species and searched for correlations between r-protein amino acid content and factors such as assembly binding order, environmental growth temperature, protein size, and contact with ribosomal RNA rRNA in the 30S complex.

We find r-proteins have a significantly high percent of positive residues, which are highly represented at rRNA contact sites. An inverse correlation between the percent of positive residues and r-protein size was identified and is mainly due to the content of Lysine residues, rather than Arginine. Nearly all r-proteins carry a net positive charge, but no statistical correlation between the net charge and the binding order was detected. Thermophilic high-temperature r-proteins contain increased Arginine, Isoleucine, and Tyrosine, and decreased Serine and Threonine compared to mesophilic lower-temperature , reflecting a known distinction between thermophiles and mesophiles, possibly to account for protein thermostability.

However, this difference in amino acid content does not extend to rRNA contact sites, as the proportions of thermophilic and mesophilic contact residues are not significantly different. Given the significantly higher level of positively charged residues in r-proteins and at contact sites, we conclude that ribosome assembly relies heavily on an electrostatic component of interaction.

However, the binding order of. On the control of ribosomal protein biosynthesis in Escherichia coli.

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These strains have a temperature-sensitive valyl-tRNA synthetase. Starvation was obtained following transfer of the cells to non-permissive temperature. Ribosomal proteins were obtained by treatment of either total lysates of freeze-thawed lysozyme spheroplasts or ammonium sulphate precipitate of ribosomes , with acetic acid. Differential labelling of the ribosomal proteins was observed in both strains: Moreover ribosomal proteins were found as stable during starvation as total protein. It is thus concluded that in starving cells individual ribosomal proteins are not synthesized at equal rates.

This indicates that the synthesis of ribosomal proteins is not only under the control of the rel gene. Post-transcriptional regulation of ribosome biogenesis in yeast. Full Text Available Most microorganisms are exposed to the constantly and often rapidly changing environment. As such they evolved mechanisms to balance their metabolism and energy expenditure with the resources available to them. When resources become scarce or conditions turn out to be unfavourable for growth, cells reduce their metabolism and energy usage to survive.

One of the major energy consuming processes in the cell is ribosome biogenesis. Unsurprisingly, cells encountering adverse conditions immediately shut down production of new ribosomes. It is well established that nutrient depletion leads to a rapid repression of transcription of the genes encoding ribosomal proteins, ribosome biogenesis factors as well as ribosomal RNA rRNA.

However, if pre-rRNA processing and ribosome assembly are regulated post-transcriptionally remains largely unclear. We have recently uncovered that the yeast Saccharomyces cerevisiae rapidly switches between two alternative pre-rRNA processing pathways depending on the environmental conditions. Our findings reveal a new level of complexity in the regulation of ribosome biogenesis. In every cell, protein synthesis is carried out by the ribosome , a complex macromolecular RNA-protein assembly.

Decades of structural and kinetic studies have increased our understanding of ribosome initiation, decoding, translocation and termination. Yet, the underlying mechanism of these fundamental processes has yet to be fully delineated. Hence, the molecular basis of regulation remains obscure. Here, single-molecule fluorescence methods are applied to decipher the mechanism and regulatory roles of the multi-step process of directional substrate translocation on the ribosome that accompanies every round of protein synthesis. In Chapter 1, single-molecule fluorescence resonance energy transfer smFRET is introduced as a tool for studying bacterial ribosome translocation.

Chapter 2 details the experimental methods. In Chapter 3, the elongation factor G EF-G -catalyzed movement of substrates through the ribosome is examined from several perspectives or signals reporting on various degrees of freedom of ribosome dynamics. Two ribosomal states interconvert in the presence of EF-G GDP , displaying novel head domain motions, until relocking takes place.

In Chapter 4, in order to test if the mentioned fluctuations leading to relocking are correlated to the engagement of the P-site by the peptidyl-tRNA, the translocation of miscoded tRNAs is studied. Severe defects in the relocking stages of translocation reveal the correlation between this new stage of translocation and P-site tRNA engagement. Full Text Available Cisplatin is a clinically important chemotherapeutic agent known to target purine bases in nucleic acids.

All of these RNAs play vital roles in the cell, such as catalysis of protein synthesis by rRNA, and therefore serve as potential drug targets. This work focused on platination of two highly conserved RNA hairpins from E. Chemical probing results also showed platination-induced RNA structural changes. These findings reveal solvent and structural accessibility of sites within bacterial RNA secondary structures that are functionally significant and therefore viable targets for cisplatin as well as other classes of small molecules.

Identifying target preferences at the nucleotide level, as well as determining cisplatin-induced RNA conformational changes, is important for the design of more potent drug molecules. Furthermore, the knowledge gained through studies of RNA-targeting by cisplatin is applicable to a broad range of organisms from bacteria to human. Using recently developed replicon mapping techniques, we have analyzed the replication of the ribosomal DNA in Saccharomyces cerevisiae.

The results show that i the functional origin of replication colocalizes with an autonomously replicating sequence element previously mapped to the. Ribosome slowed by mutation to streptomycin resistance. The effect of mutation to streptomycin resistance on the speed of polypeptide elongation in Escherichia coli was investigated. Translation speed was determined by measuring the time required for the first newly synthesized..

The results showed that ribosome speed is not a fixed parameter inherent to the protein synthetic apparatus, but a variable determined by the kinetics of translation and ultimately by the structure of the ribosome. Defective ribosome assembly in Shwachman-Diamond syndrome. Shwachman-Diamond syndrome SDS , a recessive leukemia predisposition disorder characterized by bone marrow failure, exocrine pancreatic insufficiency, skeletal abnormalities and poor growth, is caused by mutations in the highly conserved SBDS gene.

Here, we test the hypothesis that defective ribosome biogenesis underlies the pathogenesis of SDS. We create conditional mutants in the essential SBDS ortholog of the ancient eukaryote Dictyostelium discoideum using temperature-sensitive, self-splicing inteins, showing that mutant cells fail to grow at the restrictive temperature because ribosomal subunit joining is markedly impaired. Remarkably, wild type human SBDS complements the growth and ribosome assembly defects in mutant Dictyostelium cells, but disease-associated human SBDS variants are defective.

SBDS directly interacts with the GTPase elongation factor-like 1 EFL1 on nascent 60S subunits in vivo and together they catalyze eviction of the ribosome antiassociation factor eukaryotic initiation factor 6 eIF6 , a prerequisite for the translational activation of ribosomes. Importantly, lymphoblasts from SDS patients harbor a striking defect in ribosomal subunit joining whose magnitude is inversely proportional to the level of SBDS protein.

These findings in Dictyostelium and SDS patient cells provide compelling support for the hypothesis that SDS is a ribosomopathy caused by corruption of an essential cytoplasmic step in 60S subunit maturation. Emerging functions of ribosomal proteins in gene-specific transcription and translation. Ribosomal proteins have remained highly conserved during evolution presumably reflecting often critical functions in ribosome biogenesis or mature ribosome function.

In addition, several ribosomal proteins possess distinct extra- ribosomal functions in apoptosis, DNA repair and transcription. Furthermore, a subset of ribosomal proteins can bind directly to untranslated regions of mRNA resulting in transcript-specific translational control outside of the ribosome itself. Collectively, these findings suggest that ribosomal proteins may have a wider functional repertoire within the cell than previously thought.

The future challenge is to identify and validate these novel functions in the background of an often essential primary function in ribosome biogenesis and cell growth. Effect of sodium fluoride on the amount of polyribosomes, single ribosomes and ribosomal subunits in a cellular slime mold, Dictyostelium discoideum. In the slime mold, Dictyostelium discoideum, when the rate of protein synthesis was decreased by NaF, free S ribosomes accumulated at the expense of polyribosomes, while S and S ribosomal subunits remained almost constant.

The same level of ribosomal subunits was also maintained in cells after incubation with cycloheximide or at the stationary phase of growth. The complete structure of the 55S mammalian mitochondrial ribosome. Mammalian mitochondrial ribosomes mitoribosomes synthesize mitochondrially encoded membrane proteins that are critical for mitochondrial function. Here we present the complete atomic structure of the porcine 55S mitoribosome at 3.

The structure of the 28S subunit in the complex was resolved at 3. The structure reveals the intersubunit contacts in the 55S mitoribosome, the molecular architecture of the mitoribosomal messenger RNA mRNA binding channel and its interaction with transfer RNAs, and provides insight into the highly specialized mechanism of mRNA recruitment to the 28S subunit. Furthermore, the structure contributes to a mechanistic understanding of aminoglycoside ototoxicity.

The ribosome carries out the synthesis of proteins in every living cell. Published by Elsevier Inc. Ribosomes slide on lysine-encoding homopolymeric A stretches. Protein output from synonymous codons is thought to be equivalent if appropriate tRNAs are sufficiently abundant. Kinetic studies in E. Translation in a cell-free expression system demonstrates that diminished output from AAA-codon-containing reporters results from premature translation termination on out of frame stop codons following ribosome sliding. The finding that ribosomes slide on homopolymeric A sequences explains bioinformatic analyses indicating that consecutive AAA codons are under-represented in gene-coding sequences.

By utilizing four fans, the cooler is able to target the entire base of the laptop. The final result is that this cooler performs, producing an actual effect on measured CPU temperature during testing. I keep going back to it, but the simple design is really the winning key. The clean edges, cool-to-the-touch aluminum top, and overall solid build quality really make the X8 great.

Of course, like every product produced, there are a few drawbacks. It should be noted that the center mm fan spins at rpm while the outer mm fan spins at rpm. This allows the center fan to move more air and prevents them from creating a resonance under normal operation. For those looking to build a silent system Deepcool includes 7v fan adaptors which will force the fans to spin at a lower peak RPM. We were never bothered by fan noise even with both fans spinning at full tilt so using the LNA will be on a per-case basis. Overclocking on a Budget The indisputable advantages that remain are universal design, simple installation and reliable retention mechanism, as well as comparatively compact size.

But, be honest, are these features really so unique these days? Very good temperature results overall. Deepcool Ice Blade Pro V2. Being Cool and Sensible. As far as aesthetics are concerned, the Gamer Storm Assassin boasts quality materials, a unique color scheme, and an imposing presence that stands above a vast share of the CPU cooler market.

The two included hydro bearing fans are built to last and certainly don't incite any complaint in the noise department. Unless you unplug any case fans in the immediate vicinity of the cooler, it's unlikely that you'll ever hear them working. The extra long cables and mm slot compatible housing of the mm fan make both of these air movers fine candidates as premium case fans.

Those with a tall set of DIMMs may run into some clearance issues with the front mm fan. However, this clash can be quickly corrected by relocating the fan to the aft end of the heatsink. Installation is a hassle free experience thanks to Deepcool's smart approach to retention bracket design and the inclusion of rubber clips to keep evasive fasteners in check. When it comes to the Deepcool Assassin cooler, we have to say it is one of the better coolers we have tested this year. Ranging in the top half of thermal results, we loved how well it performed with our overclocked i7 processor with added voltage.

One particular advantage it has over the Silverstone HE01 cooler that we compared it to earlier in the review is its noise level. With the Deepcool Assassin running at full speed, we did hear it moreover the GPU fans but it was no contest, the Assassin was simply quieter. Mit der Rock Master V3. Mit einer maximalen Ausgangsleistung von ca.

Deepcool delivers a really nice product for the user who needs a quiet cooler that doesn't take up too much space along with being capable of keeping a computer cool, even with a little overclock. In our labs the ASSASSIN proved to be the most efficient air-cooled heatsink we have tested and it even came close to performing at the same level as high-end AiO water coolers with a mm radiator. This is impressive in itself, but when taking into account the fact that the ASSASSIN is quite a bit cheaper than mm AiO coolers the performance and price really make this a stand out product.

Deepcool M6 Notebook cooler The Deepcool M6 offers so many features and outstanding sound but does so at a penalty: The M6 is not heavy by any means, but the extra air space needed for the speakers and the height to provide the 10 degree angle make it impossible to fit in most notebook bags. You will also have to keep track of two more cables for when you are ready to use it. Retractable USB and audio cables would have been a nice feature to have. Just plan on keeping it in the location where you game the most.


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  6. By all means take it with you if you are staying out of town for a few days, but transporting it back and forth to work is out of the question. Starting with the tool free installation and the ease of mounting the cooling fan on to the cooler. Performance wise the GAMMAXX did fine considering the price of this cooler which is around rs at both stock and overclocked speed Gammaxx was able to provide decent temps and proves itself a good cooler upto 95w solution. The build quality of cooler is great.

    The cooler maybe not the best choice for extreme overclocking, but if you want a quiet cpu cooler that fits a narrow budget the Gammaxx will be a good option. DEEPCOOL shows that sticking to the basics can be successful when things are priced accordingly with what you are getting, more people would look to purchase these. With all the hype surrounding the Hyper series of coolers, there is a new god in the yard that is looking for attention and may just have what it takes to dethrone CM and the cooler that has sold I'm guessing close to a million coolers by now. Van de andere koelers is de Ice Blade Pro V2.

    Hij koelt net wat beter dan de Gelid Tranquilo Rev. De koeler is vrijwel exact even duur als de Hyper S en van die twee blijft onze voorkeur toch echt naar Cooler Master gaan. The NEPTWIN offers a very competent level of performance for its size and cost and has a staggering amount of flexibility between silence and maximum performance.

    The mounting mechanism is simple and effective and all the parts used seem to be of high quality and look well finished. Theinclusion of thermal paste and the fan hub are the deal sweeteners. The included fans are also very good. Finally, as always, the aesthetics are left down to your own personal preferences but I think Deepcool have done a good job in providing both a clean, and gamer style CPU cooler.

    You do not have to deal with a ton of tiny pieces as the cooler comes with minimum accessories that use the motherboard's stock holes and brackets for mounting. Even the fan was easy to mount using the wire hangers that comes with it. The overall appearance of the cooler is good as well. There isn't any flashy look here, just a simple single tower cooler with functional features.

    The fan with its blue and black theme would look nice with some of the midrange ASUS motherboards with the same color scheme. The DeepCool M6 Gaming cool pad offer two really good purposes if you ask me. The first we will comment on is the audio. My initial thoughts on the audio before even trying it was that it would be less than mediocre quality and low sound level simply because this has been my experience with small speaker systems aimed at laptop users. To my surprise, this was not the case. The audio quality was superb than that of my Sony laptop.

    The high notes were well represented while the lows were a little lacking but not by much. Then the volume level was twice powerful than those of the laptop's default speakers. This was enough to conclude that the M6 is a great product because listening to music on my laptop was not really enjoyable. Deepcool M6 Laptop cooler Kwa koeling zou ik de laptop koeler heel handig vinden, maar ik had al een laptop cooler, maar ik wou meer iets doen aan het geluid, en deze oplossing heeft Deepcool nu gevonden voor vele gebruikers, de combinatie van beide opties zijn zeer goed te noemen, en het geluid wat er uit komt is nog eens goed ook.

    The nickel plated copper base of the DeepCool Neptwin heatsink is flat in one axis and very slightly concave in the opposite. The polishing and subsequent metal plating leave it with a mirror smooth surface. At the top of the heatsink is a cover plate to hide the tips of the heatpipes from view. An exhaust fan can be attached to the aluminum fin tower if you wish, extra wire brackets are included for this but only one fan is supplied.

    Deepcool N9 Aluminum Notebook Cooler Although it did not provide a huge amount of direct cooling to the laptops, there was a measurable drop. Certainly the materials and design of the cooling pad minimizes the direct transfer of heat from the laptop bottom making it comfortable to position on your lap, should you be required to do so. Deepcool M3 Notebook Cooling Pad The shape of the cooling pad provides a comfortable angle for typing on a surface like a counter or a table and it is large enough to fit on your lap comfortably. The fan is very quiet, and has a variable speed wheel on the side of the cooling pad.

    I also like that it is very light, but sturdy enough that I would toss it in my luggage for a trip. The fans are actually pretty good. Even when ramping up under load they were mostly inaudible. The splitter for the fans is quite nice, both aesthetically and functionally. Big plus for including that. The performance is great for an air cooler. Really would never have expected to achieve a 4. According to Frostytech's real world sound measurements, the heatsink produces between dBA noise, making it pretty moderate on the sound front. As with most tower heatsinks, a set of extra wire fan clips are supplied so users who wish to install a rear-mounted fan can run both at low speeds.

    The Ice Wind Pro also incorporates some good improvements to DeepCool's manufacturing process, not the least of which is a perfectly flat machined base plate. It's particularly surprising with exposed heatpipe base heatsinks, but some heatsinks makers still just swag and lightly sand the base. Coldblooded Killer of Heat Deepcool Assassin is yet another super-cooler with very high cooling efficiency, two high-quality fans and fully universal and reliable retention.

    At the time of writing; however, we were unable to find any retailers that currently sell this particular cooler. It also has a two-year warranty, which is a nice bonus. Se le puede incluir un segundo ventilador trasero para ayudar en la tarea. DeepCool FrostWin puesto a prueba The S40 is a nice cooler. The looks are classy, not too flashy, it is built nice and will mount up to any of the current sockets from either Intel or AMD.

    The twin fans were barely audible over the case fans in the Corsair D test case and as long as you don't have exceedingly tall RAM modules, you won't run into clearance issues in the vast majority of cases. The focus on cooling efficiency over any kind of "bling" might not be to everyone's liking, but personally it appealed to me more because of that trait. This time, appearances were not deceiving; ASSASSIN reached about the same level of cooling performance that we saw on the best air coolers we tested to date.

    It is also relatively quiet for a high-end CPU cooler. The cooler is beautiful, well-made, has a solid mounting system, and looks nice inside the case. Its strengths are pretty obvious really: CPU cooler group test review with K As you can see, the temperatures are bumped up higher quickly. Yes the Core i7 K is a seriously nasty product when tweaked. My rule of thumb is simple, if the processor can stay under or at 75 Degrees for a long period of time, you should be okay.

    Optimal would be under 70 Degrees C. We see the two low-profile coolers and the Gelid solutions run into cooling performance issues fast, heck they are not made for this class of processor and overclock. Also, the bigger part of 35 EUR coolers like the Scythe products run out of juice fast. The last one offers great value as such.

    DeepCool Multi Core X6 DeepCool's Multi Core X6 has a neat and inviting design, and it is possible to connect one extra unit via the USB socket on the side, and the ability to set the fan speed individually in pairs. The design is sexy and inviting.

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    I find it very smart with the way you can adjust the fans speed. The price can by no means be better, with a price of ,- DKK. DeepCool's Multi Core X6 is available here. This lower cost comes at a price of a bit of ear sanity though, as the Ice Blade Pro V2. I have the feeling that it would perform better on a larger CPU like a Sandy Bridge-E or a Bulldozer, because of the size of the base and how the heat pipes are positioned, but it still performed adequately on the relatively tiny Sandy Bridge i7 K. If you like blue lighting and don't want to spend an arm and a leg to keep your reasonably-overclocked CPU cool then the Ice Blade Pro V2.

    DeepCool Ice Blade Pro v2. This is another good suggestion for a reliable and nice cooler, and it has a very low noise level, it is also super easy to install, so everyone should be able to handle the mounting of this cooler. All in all, this cooler fully comparable with the other top coolers on the market. The performance is top notch and deliver the goods, it cools well and is also very quiet and that is a big plus with me.

    The quality is good, there is nothing that is loose or seems fragile, mounting kit for both cooler and the fan also seems nice and sturdy. Deep Cool Ice Blade Pro v2. It is a brilliant product for a small price that everyone should be able to overcome. From a performance stand point we feel that DeepCool really hit the mark with the Neptwin cooler.

    While we tested this cooler with only the 2 fans provided, they have included hardware for a 3rd fan installation. This can yield slightly better performance, but from our past experience it may yield maybe a c at best; however, you will generate more noise from its 3rd fan which may or may not be a good thing for the user. Overall we feel that the DeepCool Neptwin Cooler provides excellent results. Even with our processor overclocked it proved capable of maintaining acceptable operating temperature. We think DeepCool really has something great here and its outstanding aesthetic appeal really pushes it over the top.

    Of course, take note that larger coolers like this one tend to block the RAM Dimms and this one is no exception. The Deepcool Neptwin is a very well-made, high-quality cooler. It is beautiful and seems to be very sturdy. It has good cooling performance combined with quiet operation. The Neptwin also has a flexible installation. You can install it with two fans as we did, but you can install only one fan at the middle if you experience compatibility issues with memory modules , with one fan at the middle and the other one at the rear side, or even with three fans.

    You can also, of course, replace the stock fans for more powerful ones sacrificing the silence for better performance. Because of its good look, quality, low noise level, and performance, the Deepcool Neptwin gets the Hardware Secrets Silver Award. Having tested the Deepcool Neptwin a while, I can not help but say that this is a very good product. It keeps its goal of low temperatures on my processor and in addition to that the fans are quiet so I can not be anything but happy.

    The size is perfect for my use, and by 12 heatpipes everything becomes absolutely gold end well. Deepcool will no doubt have praised for doing a good job with this product. I am looking forward to look at more products from this manufacturer. The installation is easy and reliable, which is a good characteristic for a tower cooler. It could probably perform even better if you install a second fan, or replace the quiet fan that comes with it for a stronger and louder one. Test plaque refroidissant pour portable M3 de chez Deepcool Offering comprehensive and maximum function possible health cooled plate M3 in Deepcool offers everything one can expect from it: USB hub, speaker and fan adjustable.

    It is nice to be able to listen to music and watch a movie without having to compromise on the sound! Deepcool M3 — Test This is really a very useful accessory to the extent that it avoids the inconvenience to the heating of the room, and especially the damage that may be caused to the processor and all components in a heated too high. Not to mention the hard reset full game that are really, really heavy. Deep Cool M3 Laptop cooler! Foruten dette er produktet bra. In the performance arena of the Assassin, we feel this cooler did a great job keeping our i5 k and AMD FX cool.

    With the new Intel architecture for Ivy-Bridge we have seen many reports of high idle and load temps. However with the Deepcool Gamer Storm Assassin it performed quite well even with our overclock settings on higher then normal voltages. For our AMD platform we were quite surprised at its performance, as we have not seen may air coolers able to stay under 52c under OC settings.

    The Assassin performed very closely to our custom waco set-up. This is fantastic considering the cost of water cooled set-up compared to air cooled. From the design and performance Deepcool developed a unique and high quality cooler. Viel Leistung zum fairen Preis! DeepCool Fiend Shark Qualitativ gibt er ein gutes, wenn auch nicht perfektes Bild, ab. So kann er sicher auch Silent-Enthusiasten zufrieden stellen. Twin Tower Highend Cooler! The thing that impressed me the most about this cooler, believe it or not, was its installation process.

    It took only minutes to go from having no cooler to having this one installed. It's the first of its kind that is done this way and I hope other manufacturers take a step for lighter-end coolers that can get away without a heavy-duty mounting mechanism. The base is exceptionally flat and the grooves at the heat pipe-to-base interface are almost non-existent.

    The fan is quiet and the fan mounting clips are sturdy; the included rubber vibration dampers on the fans are also a nice touch. I wouldn't hesitate to recommend this cooler for anyone in need of a mid-range, lightweight, and simple cooler. Teste do Cooler Deepcool Gammaxx Por isso, leva nosso selo Produto Recomendado.

    The Gammaxx is one of the quietest CPU cooler that we have tested, even at full load, while maintaining a decent cooling performance. The strong point of this cooler, however, is that it is inexpensive. This cooler may not be the best choice for the extreme overclocking enthusiast, but if you want a quiet CPU cooler that fits a narrow budget, the Gammaxx is a good option. For its good cooling performance with quiet operation, the Deepcool Gammaxx receives our Silver Award.

    If the manufacturer had included a back plate and a more robust installation system, maybe it would deserve the Golden one. The cooling prowess on the Deepcool Frostwin is excellent for a cooler of this size. Likely most importantly, the Frostwin is nowhere near the price of those premium coolers. Deepcool Ice Warrior Deep Cool Ice Matrix Alors que penser de ce Deep Cool Ice Matrix ? Deepcool's Ice Matrix , like its little brother the , is a great looking tower cooler that installs easily and comes with a quiet fan and a lot of nice accessories.

    It cools a bit better than the , but I expected a more significant performance increase. Granted, the is very quiet, even at its maximum fan speed Deepcool Ice Matrix Gamers and overclockers alike will enjoy this cooler. Yes, each cooler copes with our six-core CPU working at 4. The support for multiple platforms and the simple installation procedure of the Ice Matrix and the Trinity do not constitute a special advantage today.

    The new coolers are not very quiet, either but we must note that the Trinity is somewhat quieter than the Ice Matrix at the same fan speed. However, some users may want to buy one of these two products just to have a piece of the Matrix in their computers Read More.. The Deepcool Ice Matrix has a high-end looks, but a mainstream performance. It looks very nice with its narrow design and the beautiful mm blue rubber-cushioned fan.


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    6. Installation was easy, and it is also a quiet cooler, just like other Deepcool coolers we tested recently. The Deepcool Ice Matrix receives the Hardware Secrets Bronze Award, thanks to its good performance, low noise level, and great looks. It does a good job of keeping the graphics card cool while adding only the bare minimum of additional noise thanks to its super quiet fans. I'm also very impressed at the quality of the design that went into the V All aspects of the V, whether it be the heatsink, the fans, the instructions, and even the packaging are designed well.

      But again, I can't say enough how quiet these fans are and I'm disappointed I don't have the proper equipment to provide a more in-depth sound analysis. It performed as good as the best video card cooler we tested so far, while being quieter than it. Por el contrario, este no es el disipador de mayor rendimiento que hemos probado, pero no se ha quedado nada lejos de los mejores.

      The results speak for themselves: Deepcool V is one of the quietest VGA coolers available today. Its high-quality fans with anti-vibration coating make the cooler exceptionally quiet at up to RPM and acoustically comfortable at up to RPM. Therefore, Deepcool V will be a perfect choice for the owners of quiet systems. Deep Cool's Ice Blade Pro provides very good cooling performance for its modest price tag. You will need to spend a bit more on a second fan to get the most out of it. Even then, it is a still an excellent buy for overclockers on a budget, compared to more expensive brand-name heatsinks.

      Deepcool Fiend Shark Der UF besteht aus den gleichen Materialien, wie der kleinere er und kommt auch mit dem gleichen Lieferumfang daher. Die Drehzahl ist jedoch angepasst worden und wird mit rpm angegeben. Bei rpm ist sie sogar auf dem Niveau des UF bei voller Drehzahl. Auch hier kann man den mitgelieferten 7V-Adapter problemlos verwenden. Scheinbar ist beim verwendeten Lager noch Optimierungspotential vorhanden. The Ice Blade Pro uses a very common and conservative design, which is simple, inexpensive to build, and efficient, bringing a very good cooling performance.

      The blue LEDs give the fan a very nice looks, and even under full load, it was not too loud. I also like that DeepCool did not try and add a ton of extras on to the N8 like many manufacturers have done with notebook coolers. Deepcool N8 Review While the Deepcool N8 might not have as many frills as some of the other coolers I have reviewed, it does provide a professional, crisp, look that many other coolers do not have.

      This, bundled with decent cooling ability, provided by the aluminum base as well as the two large fans, as well as a USB hub make it a good choice for anyone who wants to give out a professional appearance while on the go. Additionally, its smaller size allows much more desk space to be used for other things while still allowing a 15" laptop to be used with it. Finally, the tilt of the N8 allows the user to derive ergonomic benefits by placing the laptop's built in keyboard at a slant. Overall, I would recommend the N8 to anyone who is looking for a professional looking laptop cooler that can still get the job done.

      Les performances sont bonnes et suffisantes pour palier la chaleur des gros processeurs mais on reste en de? Deepcool Ice Matrix Review The Ice Matrix will still be a good buy for someone who wants something quiet, small, and inexpensive. The Ice Matrix certainly meets everything in that category. At full speed, I can't pick out the noise from the fan over the other fans in my case, which is a good thing.